However, an important issue concerns the details of the mode of delivery of steroid hormones to their target cells. In the plasma compartment, the steroid hormones move through the circulatory system bound to their partner transport protein. This chapter summarizes some key concepts that should be considered during development and validation of steroid hormone mass spectrometry assays, focusing primarily on LC-MS/MS assays. However, due to the structural similarities seen between steroid hormones, it is imperative that sufficient interference testing be undertaken to ensure that no cross-reactivity exists between the different hormones in the mass spectrometry method. Mass spectrometry offers the capability of this measurement range and is also precise and accurate when assays are developed and calibrated correctly. The methods used to measure these hormones require a large dynamic measurement range, but also in many cases need to be capable of detecting down to pg/mL concentrations.
Steroid hormones are commonly measured in patients for the diagnosis, treatment, and prevention of hormone-related diseases in men, women, and children. French, in Mass Spectrometry for the Clinical Laboratory, 2017 Abstract Among men, higher total testosterone was associated with higher logHDL and logLDL particle size, and lower logtriglycerides and logVLDL, but these associations were partially attenuated with adjustment for adiposity and HOMA-IR.Īmong Asian Indians, SHBG is associated with more favorable lipid subfraction concentrations, independent of hepatic and visceral fat.J.C. Relationships between sex steroids and lipid subfractions were not significant among women. Among men, lower logSHBG was also associated with smaller logLDL peak diameter size and higher logtriglycerides and logVLDL, even after adjustment for HOMA-IR and adiposity. Among women, lower logSHBG levels was significantly associated with lower logmedium LDL and logsmall LDL concentrations even after consideration of visceral and hepatic adiposity and insulin resistance as represented by the homeostasis model assessment of insulin resistance (HOMA-IR). Multivariable regression models estimated the association between sex hormones with lipoprotein subfractions before and after adjustment for adiposity.Īmong women, lower logSHBG levels were associated with smaller logLDL particle size and higher logtriglycerides, logVLDL, and logIDL, although these associations were attenuated with adjustment for visceral adiposity in particular. Intra-abdominal adiposity was assessed by computed tomography. Lipoprotein subfractions including low-density lipoprotein cholesterol (LDL), very low-density lipoprotein cholesterol (VLDL), and intermediate density lipoprotein (IDL) were assessed by ion mobility spectrometry. We used data from 42 women and 57 Asian Indian men who did not use exogenous steroids or lipid-lowering medications.
Our objective was to determine if associations are independent from adipose tissue area among Asian Indians. Estradiol, testosterone (T), and sex hormone binding globulin (SHBG) levels are associated with lipid subfractions in men and women.